Molecular Biotechnology, IFM
Jutta Speda, Dipl.-Ing.(FH) Biotechnologie
Division of Molecular Biotechnology
Department of Physics, Chemistry and Biology
SE-581 83 Linköping
Office: +46 (0)13 286640
Nanoparticle induced functionalization of designed polypeptides
The functionalization of de novo polypeptides gives rise to a wide application in industry and science. Special designed peptides are capable to form helical secondary structures upon addition of silica nanoparticles, even resulting in switch-like characteristics that refer to an attached catalytic side for ester hydrolysis.
In my studies, the basic peptide design is further varied to investigate the influence of rather bulky pyrene-labeled cysteine side chains onto the polypeptide secondary structures. Peptides exhibiting one or two labels in different positions are examined with respect to associated changes in silica nanoparticle induced secondary structures. Additionally the effects of secondary structure formation on fluorescent emission spectra are investigated as pyrene-labels tend to form excimers upon convergence.
The obtained results are important to understand the influence of polypeptide modifications on secondary structure for further optimizations.
Prof. Bengt-Harald "Nalle" Jonsson
Last updated: 2011-12-06