Assays and Techniques

Welcome to Forum Scientiums list of Assays and Techniques, where Forum Scientium maintains a list of it's members' technical and methodological knowledge in different life-science fields.

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|| Cell Biology ||Microscopy || Cell Culture || Animal Models || Molecular Biology || Protein Assays || Proteomics ||
|| Chemistry || Microfabrication || Surface Analysis || Biosensors || Statistics ||

Cell Biology

Flow cytometry and Cell-sorting

Instruments: FACSCalibur, LSR, FACSAria, FACSCanto, Gallios (Core Facility)

Applications: Detection of fluorescence-labeled cells, 2-way, 4-way or single-cell sorting

Cell fractionation

Virus Construction/Transduction

Applications:

Siri Fagerholm (adenovirus)
Camilla Halvarsson (retrovirus, lentivirus)
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Immunohistochemistry

Expression of fluorescent fusion proteins

In-Situ Hybridization

Magnus Baumgardt (In vivo)
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β-radiation counting - Scintillation

Instruments: Scintillation counters, in liquid or membranes (Beckman-Coulter, Wallac).

Applications: H³-Thymidine incorporation for proliferation assays.

Mikael Pihl (H³-Thymidine, in membranes, Wallac)
Isa Lindgren (Liquid Scintillation, Beckman-Coulter)
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γ-radiation counting - Scintillation

Instruments:

Applications:

Microscopy

Confocal microscopy

Magnus Baumgardt (Zeiss Confocals)
Thommie Karlsson (Zeiss VivaTome)
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Fluorescence Microscopy

Live Cell Microscopy

Applications: High speed time-lapse confocal/epi fluorescence imaging

Total Internal Reflection Microscopy (TIRF)

Differential Interference Contrast Microscopy (DIC)

Epi Fluorescence Microscopy

Dual Excitation Ratio Imaging (Fura-2)

SEM - Scanning Electron Microscopy

Instruments: LEO 1550 Gemini (Responsible: Thomas Lingefelt)

Application: Imaging features >50nm, EDX spectra for element analysis

AFM - Atomic Force Microscopy

Instruments: Dimension 3100, Enriroscopy (Responsible: Jörgen Bengtsson)

Applications: Imaging features >1nm, also surface force interactions.

Cell culture

Adherent cells

Thommie Karlsson (Cell lines)
Karin Magnusson
Camilla Halvarsson
Linda Åkerman
Sofie Sundberg (Neuronal and glial cells)
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Non-Adherent cells

Mikael Pihl (T-lymphocytes, T-lymphocyte clones)
Siri Fagerholm (Primary Adipocytes, Cell lines, Stem cells)
Ia Brännmark (Primary Adipocytes, Cell lines)
Thommie Karlsson (Cell lines)
Camilla Halvarsson
Linda Åkerman (T-lymphocytes)
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Transfection

Micro-injection

Electrophysiology

Sofie Sundberg (Patch clamp)
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Animal Models

Drosophila Melanogaster

Mus Musculus

Camilla Halvarsson (Genetic modification of mice, Transgenesis, Gene Targeting)
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Molecular Biology

At HU, several instruments are available at Cellkärnan, Cell Biology building floor 9 (Core Facility)

PCR

Instruments: Thermal Cyclers

Applications: Genotyping assays

Realtime PCR / quantitative PCR

Instruments: 7500 Fast, 7900 Fast Real-Time PCR System, Corbett

Applications: Gene Expression assays, Genotyping assays

Mikael Pihl (7500 Fast)
Isa Lindgren (Corbett)
Camilla Halvarsson
Linda Åkerman (gene expression arrays, 7900 ht)
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DNA Sequencing

Instruments: Megabace

Restriction Enzyme Cleavage

Applications:

Transformation

Molecular Cloning

Protein Assays

SDS-PAGE

Applications:

Western Blot

Applications:

ELISA

Instruments: Versamax microplate reader

Applications: Protein quantification

Ia Brännmark
Linda Helmfors (Meso Scale Discovery)
Maria Jonsson (Meso Scale Discovery)
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Immuno Precipitation

Applications:

Protein purification

Proteomics

Mass Spectrometry

Instruments: Voyager DE Pro MALDI-TOF, QSTAR, Esquire HCT Ultra (at HU: Cellkärnan (Core Facility))

Applications:

In-gel digestion

Applications:

Peptide Synthesis

Instruments: Pioneer Synthesis System

Applications: Peptide Synthesis

NMR - Nuclear Magnetic Resonance

Leif Johansson (small organic molecules)
Alexandra Ahlner (proteins)
Rozalyn Simon
Mattias Tengdelius
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Chemistry

Electrophoresis (SDS, agarose, protein, DNA)

SPR - Surface Plasmon Resonance

Instruments: Biacore (GE), in-house SPR for imaging

Applications: Biomolecular interaction analysis

HPLC - High Pressure/Performance Liquid Chromatography

Instruments: Wave (at HU: Cellkärnan (Core Facility))

Applications: Identify, quantify and separate compounds

Åsa Jufvas (proteins)
Robert Selegård (peptides)
Mikael Pihl (denaturing HPLC, DNA/Molecular Biology)
Sara Helander
Leif Johansson (small organic molecules)
Jutta Speda (peptides)
Mattias Tengdelius
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CD - Circular Dichroism

Spectral Imaging

XPS - X-ray Photoelectron Spectroscopy

Applications: Elementary analysis, Chemical surroundings, Bonds

Measurment of Relaxation

Instruments: Minispec mq60 NMR analyzer

Applications: Measuring relaxation times, T1 & T2

Fluorescence Spectroscopy

Instruments: Fluoromax spectrofluorometer

Applications: Excitation and Emission spectra ~200-800nm, inter-protein binding affinity measurements

DLS - Dynamic Light Scattering

Instruments:

Application: Hydrodynamic radius of particles in solution

Cyclic Voltammmetry

Instruments: Autolab

Applications: Electrochemical measurments

Soft Lithography

Applications: Polymerisation of PDMS for contruction of microfluidic systems

QCM-D - Quartz Crystal Microbalance with Dissipation monitoring

Applications: Characterization of the formation of thin films (nm) onto surfaces in liquid.

Programming

Instruments: LabView software

Applications: Creating Steering programs for instruments

Electropolymerization

Applications: Polymerization of conducting polymers using electrochemical reactions

Instruments: Autolab

Chemical Polymerization

Applications: Polymerization of conducting polymers using chemical oxidation

UV-polymerization

Isoelectric focusing

Isothermal Titration Calorimetry

Differential Scanning Calorimetry

Microfabrication

Photolithography

Applications: Microfabrication and patterning of substrates

Reactive Ion Etch

Applications: Surface tratment with a chemically reactive plasma

Electrical characterization

Applications: IV scans and sourcing currents

Surface Analysis

Null Ellipsometry

Instruments: AutoEl (Rudolph Research), EP3 (nanofilm)

Applications: Surface thin film thickness

Contact Angle Goniometry

Instruments: CAM 200 (KSV)

Applications: Surface wettability

Infrared Spectroscopy - Transmission mode

Instruments: Vertex 70 (Bruker)

Applications: Sample composition/identification of powder or liquid

Infrared Spectroscopy - IR Microscope

Instruments: Hyperion 3000 (Bruker)

Applications: Chemical Composition analysis in local area

Infrared Reflection Absorption Spectroscopy

Instruments: Bruker IFS66

Applications: Surface structure e.g. molecular conformation, orientation, and molecular packing in the SAMs

Raman Spectroscopy

Instruments: FRA106 (Bruker)

Applications: Chemical composition, complimentary to IR spectroscopy

Dektak

Applications: Measure height profiles on surfaces

Biosensors

Photoplthysmography

Applications: Detects pulsatile changes in blood volume in different tissues and depths

Laser Doppler Flowmetry

Instruments: PeriFlux 5000

Applications: Microcirculatory assessment of skin viability

Statistics

Software

GraphPad Prism

Genex

Applications: Gene expression analysis

Statistical Tests

Mathematical Modelling

Page responsible: jonathan.rakar@liu.se
Last updated: Mon Jul 30 16:53:36 CEST 2012